Vitamin C Research Today is a free monthly online journal that collates and summarizes the latest research about Vitamin C, including details on benefits, dosage, supplements, information.

Direct fluorimetric determination of ascorbic acid by the supramolecular system of AA with beta-cyclodextrin derivative.

Wang L, Zhang L, She S, Gao F

College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. zhangli322@etang.com

We established a simple and selective fluorimetric method for the determination of ascorbic acid (AA) with the novel mono-[6-N(4-carboxy-phenyl)]-beta-cyclodextrin (ACD). The method is based on the fluorescence intensity of ACD decreases as the ACD-AA supramolecular complexes form. The fluorescence intensity is measured at excitation and emission wavelengths of 270 and 352 nm, respectively. Under optimum condition, a linear relationship is obtained between the fluorescence intensity and the concentration of AA in the range of 0.05-8.0 microgml(-1). The detection limit is 0.012 microgml(-1). The method has been applied to the direct analysis of AA in real samples with satisfactory results.

Published 26 July 2005 in Spectrochim Acta A Mol Biomol Spectrosc, 61(11): 2737-40.
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